yum install -y gcc python3-devel

yum -y install zlib zlib-devel libffi-devel

git clone https://github.com/khuang28jhu/bs3.git

cd bs3

pip3.9 install matplotlib -i https://mirrors.aliyun.com/pypi/simple/ --no-deps

pip3.9 install pysam -i https://mirrors.aliyun.com/pypi/simple/ --no-deps

python3.9 -m pip install 2to3 

cd bs3上一级目录

2to3 --output-dir=bs3-version3 -W -n bs3

vim bs3-build

vim bs3-align

chmod +x bs3-build

chmod +x bs3-align

bs3-bulid -h

import bcl2fastq

镜像拉取

docker pull swr.cn-east-3.myhuaweicloud.com/hpc/bs3:1.0