一、软件介绍：

Alfred：BAM 比对统计、特征计数和特征注释工具

开发语言：C++

源码地址：https://github.com/tobiasrausch/alfred/archive/refs/tags/v0.2.7.tar.gz

二、安装步骤：

1、依赖安装：

yum install wget tar gcc-c++ boost-devel autoconf automake bzip2-devel xz-devel

2、alfred安装：

cd /path/to/alfred

wget https://github.com/tobiasrausch/alfred/archive/refs/tags/v0.2.7.tar.gz

tar xf v0.2.7.tar.gz

cd alfred-0.2.7/src/htslib

wget https://github.com/samtools/htslib/releases/download/1.18/htslib-1.18.tar.bz2

tar xf htslib-1.18.tar.bz2 --strip-components=1

cd /path/to/alfred/alfred-0.2.7

make

make install

3、软件验证：

cd /path/to/alfred/alfred-0.2.7/bin

./alfred -h

四、故障排查：

1、make: g++: Command not found

解决方案：yum install gcc-c++

2、src/alfred.cpp:8:10: fatal error: boost/program_options/cmdline.hpp: No such file or directory

解决方案：yum install boost-devel

3、src/alfred.cpp:18:10: fatal error: htslib/sam.h: No such file or directory

解决方案：

cd /path/to/alfred/alfred-0.2.7/src/htslib

wget https://github.com/samtools/htslib/releases/download/1.18/htslib-1.18.tar.bz2

tar xf htslib-1.18.tar.bz2 --strip-components=1

4、/bin/sh: autoreconf: command not found

解决方案：yum install autoconf automake

5、configure: error: libbzip2 development files not found

解决方案：yum install bzip2-devel

6、configure: error: liblzma development files not found

解决方案：yum install xz-devel