环境要求

硬件要求

硬件要求如表1所示。

表1 硬件要求

项目

说明

CPU

鲲鹏920处理器

软件要求

软件要求如表2所示。

表2 软件要求

项目

版本

下载地址

CMake

3.17.3

https://cmake.org/files/v3.17/cmake-3.17.3.tar.gz

Boost

1.66.0

https://boostorg.jfrog.io/artifactory/main/release/1.66.0/source/boost_1_66_0.tar.gz

salmon

1.4.0

https://github.com/COMBINE-lab/salmon/archive/v1.4.0.tar.gz

Jellyfish

2.2.10

https://github.com/gmarcais/Jellyfish/releases/download/v2.2.10/jellyfish-2.2.10.tar.gz

htslib

1.11

https://github.com/samtools/htslib/archive/1.11.tar.gz

Samtools

1.11

https://github.com/samtools/samtools/archive/1.11.tar.gz

Bowtie2

2.3.5.1

https://netix.dl.sourceforge.net/project/bowtie-bio/bowtie2/2.3.5.1/bowtie2-2.3.5.1-source.zip

sse2neno

master

https://github.com/jratcliff63367/sse2neon/archive/master.zip

SIMDe

v0.7.0-rc-1

https://github.com/simd-everywhere/simde/archive/v0.7.0-rc-1.tar.gz

Python

3.8

https://www.python.org/ftp/python/3.8.0/Python-3.8.0.tgz

Cython

0.29.21

https://files.pythonhosted.org/packages/6c/9f/f501ba9d178aeb1f5bf7da1ad5619b207c90ac235d9859961c11829d0160/Cython-0.29.21.tar.gz

numpy

1.19.4

https://files.pythonhosted.org/packages/c5/63/a48648ebc57711348420670bb074998f79828291f68aebfff1642be212ec/numpy-1.19.4.zip

Trinity

2.11.0

https://github.com/trinityrnaseq/trinityrnaseq/archive/v2.11.0.tar.gz

Inchworm

master

https://github.com/trinityrnaseq/Inchworm/archive/master.zip

Chrysalis

master

https://github.com/trinityrnaseq/Chrysalis/archive/8ef13ed1fa618186ec3dc04522115c99fdfacda2.zip

seqtk-trinity

master

https://github.com/trinityrnaseq/seqtk-trinity/archive/f5e229dc164823c3ef14e8b8e2ba7d84c8d80e6c.zip

bamsifter

master

https://github.com/trinityrnaseq/bamsifter/archive/f034f247d5fa1fd9847c390d99bfd918da7d7739.zip

算例1

SRR3050898_1.fastq

http://ftp.sra.ebi.ac.uk/vol1/fastq/SRR305/008/SRR3050898/SRR3050898_1.fastq.gz

算例2

SRR3050898_2.fastq

http://ftp.sra.ebi.ac.uk/vol1/fastq/SRR305/008/SRR3050898/SRR3050898_2.fastq.gz

操作系统要求

操作系统要求如表3所示。

表3 操作系统要求

项目

版本

下载地址

CentOS

7.6

https://www.centos.org/download/

Kernel

4.14.0-115

包含在操作系统镜像中。