运行和验证
操作步骤
- 使用PuTTY工具，以root用户登录服务器。
- 执行以下命令运行Bowtie2获取“SAM”文件。
bowtie2 -p 6 -3 5 --local -x mm10 -1 bowtie2-2.4.1/example/reads/reads_1.fq -2 bowtie2-2.4.1/example/reads/reads_2.fq -S example.sam
结果样例显示如下所示。
10000 reads; of these: 10000 (100.00%) were paired; of these: 10000 (100.00%) aligned concordantly 0 times 0 (0.00%) aligned concordantly exactly 1 time 0 (0.00%) aligned concordantly >1 times 0.00% overall alignment rate