---
title: 运行和验证
description: "1. 使用PuTTY工具，以root用户登录服务器。"
url: https://www.hikunpeng.com/document/detail/zh/kunpenghpcs/hpcindapp/prtg-osc/kunpengbowtie2_02_0009.html
sourcePath: /source/zh/kunpenghpcs/hpcindapp/prtg-osc/kunpengbowtie2_02_0009.html
indexId: 21444b8725cd9e2c2b5654bed182ae47a11e50f1c59202a681b484e8f7bc21de69
---
# 运行和验证

#### 操作步骤

1. 使用PuTTY工具，以root用户登录服务器。
2. 执行以下命令运行Bowtie2获取“SAM”文件。

```
bowtie2 -p 6 -3 5 --local -x mm10 -1 bowtie2-2.4.1/example/reads/reads_1.fq -2 bowtie2-2.4.1/example/reads/reads_2.fq -S example.sam
```


  结果样例显示如下所示。

```
10000 reads; of these:
10000 (100.00%) were paired; of these:
10000 (100.00%) aligned concordantly 0 times
0 (0.00%) aligned concordantly exactly 1 time
0 (0.00%) aligned concordantly >1 times
0.00% overall alignment rate
```