---
title: 运行和验证
description: "1. 使用PuTTY工具，以root用户登录服务器。"
url: https://www.hikunpeng.com/document/detail/zh/kunpenghpcs/hpcindapp/prtg-osc/kunpengcufflinks_02_0011.html
sourcePath: /source/zh/kunpenghpcs/hpcindapp/prtg-osc/kunpengcufflinks_02_0011.html
indexId: 22090e9b9818907f2602855945b126579e6e582c7487f9a1575b4dadcb06a0fa71
---
# 运行和验证

#### 操作步骤

1. 使用PuTTY工具，以root用户登录服务器。
2. 执行以下命令进入源码自带的测试文件路径并运行。

```
cd
/path/to/CUFFLINKS
/cufflinks-master/external_tests/tiny_tests/2x75
```


3. 执行以下命令转换算例格式。

```
samtools view -bS accepted_hits.sam > accepted_hits.bam
```


4. 执行以下命令运行算例。

```
cufflinks accepted_hits.bam
```


  运行状态如下所示，表示Cufflinks正常结束。

```
Warning: Using default Gaussian distribution due to insufficient paired-end reads in open ranges.  It is recommended that correct parameters (--frag-len-mean and --frag-dev) be provided.
> Map Properties:
>       Normalized Map Mass: 90.00
>       Raw Map Mass: 90.00
>       Fragment Length Distribution: Truncated Gaussian (default)
>                     Default Mean: 200
>                  Default Std Dev: 80
[19:58:21] Assembling transcripts and estimating abundances.
> Processed 1 loci.                            [*************************] 100%
```