Installing Samtools

Procedure

1. Use PuTTY to log in to the server as the root user.
2. Run the following command to decompress the Samtools installation package:

   tar -xvf samtools-1.10.tar.bz2

3. Run the following command to switch to the directory generated after the package is decompressed:

   cd samtools-1.10

4. Run the following command to perform installation:

   yum install -y bzip2-devel bzip2-libs bzip2

5. Run the following command to perform configuration:

   ./configure --prefix=/path/to/SAMTOOLS

6. Run the following commands to perform compilation and installation:

   make all all-htslib
   make install install-htslib

7. Run the following commands to set the Samtools environment variables:

   export PATH=/path/to/SAMTOOLS/bin:$PATH
   export LD_LIBRARY_PATH=/path/to/SAMTOOLS/lib:$LD_LIBRARY_PATH