Compiling and Installing BLAST

Procedure

1. Use PuTTY to log in to the server as the root user.
2. Run the following command to decompress and configure the BLAST path:

   tar xzf ncbi-blast-2.9.0+-src.tar.gz
   cd ncbi-blast-2.9.0+-src
   export BLAST_TOP=`pwd`

3. Run the following command to configure the compiler:

   export CC=gcc
   export CXX=g++

4. Use an SFTP tool to upload LMDB_0.9.24.tar.gz to the /path/to/BLAST/ncbi-blast-2.9.0+-src directory.
5. Run the following command to decompress and configure the LMDB path:

   cd $BLAST_TOP
   tar -xvf LMDB_0.9.24.tar.gz
   cd lmdb-LMDB_0.9.24
   export LMDB_PATH=$BLAST_TOP/lmdb-LMDB_0.9.24

6. Run the following commands to compile and install BLAST:

   cd $BLAST_TOP/c++
   ./configure
   cd ReleaseMT/build
   make all_r -j40

7. After the installation is successful, the executable file is generated in the $BLAST_TOP/c++/ReleaseMT/bin directory. You can run the following command to view the file:

   cd $BLAST_TOP/c++/ReleaseMT/bin
   ls blastp

   blastp