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Creating a Bowtie2 Index

Procedure

  1. Use PuTTY to log in to the server as the root user.
  2. Run the following command to switch to the directory generated after the package is decompressed:
    tar -zxvf chromFa.tar.gz
  3. Run the following command to perform the redirection:
    cat *.fa > mm10.fa
  4. Run the following command to create an index:
    bowtie2-build mm10.fa mm10