Running and Verifying Bowtie
Procedure
- Use PuTTY to log in to the server as the root user.
- Run the following command to switch to the man_hg19 directory:
cd /path/to/CHROMFA/man_hg19/
- Run the following commands to decompress the case files:
gzip -d SRR742200_1.fastq.gz gzip -d SRR742200_2.fastq.gz
- Run the following command to run Bowtie to obtain the .sam file (the corresponding biological sequence text xxx.fastq is required):
bowtie -S -t -q -p 128 man_index -1 ./SRR742200_1.fastq -2 ./SRR742200_2.fastq SRR742200_out.sam
The following is an example of the result:

Parent topic: Bowtie 1.2.3 Porting Guide (CentOS 7.6)