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Running and Verifying Bowtie

Procedure

  1. Use PuTTY to log in to the server as the root user.
  2. Run the following command to switch to the man_hg19 directory:
    cd /path/to/CHROMFA/man_hg19/
  3. Run the following commands to decompress the case files:
    gzip -d SRR742200_1.fastq.gz
    gzip -d SRR742200_2.fastq.gz
  4. Run the following command to run Bowtie to obtain the .sam file (the corresponding biological sequence text xxx.fastq is required):
    bowtie -S -t -q -p 128 man_index -1 ./SRR742200_1.fastq -2 ./SRR742200_2.fastq SRR742200_out.sam

    The following is an example of the result: