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Running and Verifying Diamond

Procedure

  1. Use PuTTY to log in to the server as the root user.
  2. Run the following command to go to the case directory and decompress the case:

    cd /path/to/TESTCASE

    gzip -d human_g1k_v37.fasta.gz

    gzip -d swissprot.gz

  3. Run the following command to run the case:

    diamond blastp -p 128 -q human_g1k_v37.fasta -d swissprot -o out.tsv --very-sensitive

    After the command is executed, the following information is displayed:
    .......
    Loading reference sequences...  [0s]
    Deallocating buffers...  [0s]
    Deallocating queries...  [0.002s]
    Loading query sequences...  [0s]
    Closing the input file...  [0s]
    Closing the output file...  [0s]
    Closing the database file...  [0.003s]
    Deallocating taxonomy...  [0s]
    Total time = 538.485s
    Reported 0 pairwise alignments, 0 HSPs.
    0 queries aligned.