Running and Verification
Procedure
- Run the following command to go to the test case directory:
cd /path/to/TESTCASE
- Run the following commands to decompress the test case file packages:
gzip -d Drosophila_melanogaster.BDGP6.dna.toplevel.fa.gz
gzip -d R1.fq.gz
gzip -d R2.fq.gz
- Run the following command to rename the fa file:
mv Drosophila_melanogaster.BDGP6.dna.toplevel.fa genome.fa
- Run the following command to create a genome index:
hisat-build genome.fa genome
- Run the following command to compare gene sequences:
hisat -t -x genome -p 96 -1 R1.fq -2 R2.fq -S result.sam
If the following information is displayed, the verification is successful:

Parent topic: HISAT 0.1.6 Porting Guide (CentOS 7.6)