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Running and Verification

Procedure

  1. Run the following command to go to the test case directory:

    cd /path/to/TESTCASE

  2. Run the following commands to decompress the test case file packages:

    gzip -d Drosophila_melanogaster.BDGP6.dna.toplevel.fa.gz

    gzip -d R1.fq.gz

    gzip -d R2.fq.gz

  3. Run the following command to rename the fa file:

    mv Drosophila_melanogaster.BDGP6.dna.toplevel.fa genome.fa

  4. Run the following command to create a genome index:

    hisat-build genome.fa genome

  5. Run the following command to compare gene sequences:

    hisat -t -x genome -p 96 -1 R1.fq -2 R2.fq -S result.sam

    If the following information is displayed, the verification is successful: