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Installing Bowtie 2

  1. Use PuTTY to log in to the server as the root user.
  2. Delete the Bowtie 2 preinstalled by HUMAnN:
    rm -rf /path/to/python-3.9.6/bin/bowtie2*
  3. Download the Bowtie 2 installation package.

    http://sourceforge.net/projects/bowtie-bio/files/bowtie2/2.4.2/bowtie2-2.4.2-linux-aarch64.zip

  4. Decompress the installation package.
    unzip bowtie2-2.4.2-linux-aarch64.zip
  5. Edit the configuration file.
    1. Open the configuration file.
      vi /etc/profile
    2. Press i to enter the insert mode and add the Bowtie 2 path.
      export PATH=/home/gcc-9.3.1-2020.12-aarch64-linux/bin:/usr/local/python-3.9.6/bin:/home/bowtie2-2.4.2-linux-aarch64:$PATH
    3. Press Esc, type :wq!, and press Enter to save the file and exit.
  6. Make the configuration take effect.
    source /etc/profile
  7. Check whether Bowtie 2 is successfully installed.
    bowtie2 -h

    The installation is successful if the following information is displayed: