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Running and Verifying LASTZ

Procedure

  1. Use PuTTY to log in to the server as the root user.
  2. Run the following command to go to the CASE directory:
    cd /path/to/CASE
  3. Run the following commands to decompress the test case packages:
    gzip -d hg38.chr4.fa.gz
    gzip -d galGal3.chr4.fa.gz
  4. Run the following command to run the test case:
    time lastz hg38.chr4.fa galGal3.chr4.fa --notransition --step=20 --nogapped --format=maf > hg38_4_vs_galGal3_4-kp7265.maf
  5. Run the following command to view hg38_4_vs_galGal3_4-kp7265.maf:
    vim hg38_4_vs_galGal3_4-kp7265.maf

    Figure 1 is an example of the output result.

    Figure 1 Result example