Running and Verification

Procedure

1. Use PuTTY to log in to the server as the root user.
2. Create a test directory and switch to the directory.

   mkdir -p /path/to/ ensembl-vep-release-104.3
   cd /path/to/ ensembl-vep-release-104.3

3. Start VEP.

   ./vep -i examples/homo_sapiens_GRCh38.vcf -o output.txt –cache

   

   If ensembldb.ensembl.org cannot be connected, download the cache file (see 6) and use the offline mode.

   ./vep -i examples/homo_sapiens_GRCh38.vcf -o output.txt –cache

   

   

   If an output.txt file is generated, the execution is successful.

4. View the html file.