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Creating a Bowtie Index

Procedure

  1. Use PuTTY to log in to the server as the root user.
  2. Run the following command to switch to the CHROMFA directory:
    cd /path/to/CHROMFA
  3. Run the following command to decompress the chromFa installation package:
    tar -xzvf chromFa.tar.gz
  4. Run the following command to perform the redirection:
    cat *.fa > man.fa
  5. Run the following command to create an index:
    bowtie-build --threads 128 man.fa man_index
  6. Run the following commands to create a man_hg19 directory and save the generated man_index*.ebwt file to this directory:
    mkdir man_hg19
    mv  *.ebwt  man_hg19