Creating a Bowtie Index
Procedure
- Use PuTTY to log in to the server as the root user.
- Run the following command to switch to the CHROMFA directory:
cd /path/to/CHROMFA
- Run the following command to decompress the chromFa installation package:
tar -xzvf chromFa.tar.gz
- Run the following command to perform the redirection:
cat *.fa > man.fa
- Run the following command to create an index:
bowtie-build --threads 128 man.fa man_index
- Run the following commands to create a man_hg19 directory and save the generated man_index*.ebwt file to this directory:
mkdir man_hg19 mv *.ebwt man_hg19
Parent topic: Compiling and Installing Bowtie