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Running and Verifying FastQC

Procedure (Using CLI)

  1. Use PuTTY to log in to the server as the root user.
  2. Run the following command to create a test directory:
    mkdir -p /path/to/FASTQC/test
  3. Run the following commands to copy the test case to the test directory and switch to the test directory:
    cp B17NC_R1.fastq.gz /path/to/FASTQC/test
    cd /path/to/FASTQC/test
  4. Run the following command to decompress the test case:
    gzip -d B17NC_R1.fastq.gz
  5. Run the following command to run the test:
    /path/to/FASTQC/FastQC/fastqc -o ./ ./B17NC_R1.fastq
  6. After the execution is complete, the B17NC_R1_fastqc.html and B17NC_R1_fastqc.zip files are generated. Run the following command to check whether the files exist:
    ll

    Use a browser to open the html file and view the result.

Procedure (Using GUI)

  1. Use PuTTY to log in to the server as the root user.
  2. Run the following command to log in to the GUI:
    /path/to/FASTQC/FastQC/fastqc
  3. Click File > Open in the upper left corner and select a case.

  4. Select a test case and click Open. If the following page is displayed, the test is running.

    Figure 1 shows an example of the test result.

    Figure 1 Result example