Running and Verifying FastQC
Procedure (Using CLI)
- Use PuTTY to log in to the server as the root user.
- Run the following command to create a test directory:
mkdir -p /path/to/FASTQC/test
- Run the following commands to copy the test case to the test directory and switch to the test directory:
cp B17NC_R1.fastq.gz /path/to/FASTQC/test cd /path/to/FASTQC/test
- Run the following command to decompress the test case:
gzip -d B17NC_R1.fastq.gz
- Run the following command to run the test:
/path/to/FASTQC/FastQC/fastqc -o ./ ./B17NC_R1.fastq
- After the execution is complete, the B17NC_R1_fastqc.html and B17NC_R1_fastqc.zip files are generated. Run the following command to check whether the files exist:
ll
Use a browser to open the html file and view the result.
Procedure (Using GUI)
- Use PuTTY to log in to the server as the root user.
- Run the following command to log in to the GUI:
/path/to/FASTQC/FastQC/fastqc
- Click File > Open in the upper left corner and select a case.


- Select a test case and click Open. If the following page is displayed, the test is running.

Figure 1 shows an example of the test result.
Parent topic: FastQC 0.11.9 Porting Guide (CentOS 7.6)
