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Running and Verification

Procedure

  1. Use PuTTY to log in to the server as the root user.
  2. Check whether the installation is successful.
    cd /path/to/LTR_retriever/LTR_retriever-2.9.0
    ./LTR_retriever -h

    Information similar to the following is displayed:

  3. Start the running.
    mkdir test && cd test
    wget http://ftp.ensembl.org/pub/release-106/fasta/gallus_gallus/dna/Gallus_gallus.GRCg6a.dna.chromosome.16.fa.gz
    gunzip Gallus_gallus.GRCg6a.dna.chromosome.16.fa.gz
    mv Gallus_gallus.GRCg6a.dna.chromosome.16.fa Gallus.fa
    gt suffixerator -db genome.fa -indexname genome.fa -tis -suf -lcp -des -ssp -sds -dna
    gt ltrharvest -index genome.fa -minlenltr 100 -maxlenltr 7000 -mintsd 4 -maxtsd 6 -motif TGCA -motifmis 1 -similar 85 -vic 10 -seed 20 -seqids yes > genome.fa.harvest.scn
    LTR_retriever -genome genome.fa -inharvest genome.fa.harvest.scn

    Information similar to the following is displayed: